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T6258

Sigma-Aldrich

Triosephosphate Isomerase from rabbit muscle

Type X, lyophilized powder, ≥3,500 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:
9023-78-3
Enzyme Commission number:
5.3.1.1 (BRENDA, IUBMB)
EC Number:
232-774-2
MDL number:
MFCD00082080
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type X

form

lyophilized powder

specific activity

≥3,500 units/mg protein

mol wt

calculated mol wt 53.2 kDa

composition

Protein, 60-90% biuret

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%

storage temp.

−20°C

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General description

Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.

Application

Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).
Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.

Biochem/physiol Actions

Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.

Packaging

Package size based on protein content

Unit Definition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Sulfate-free, contains EDTA and borate buffer salts

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hydrophobic interaction chromatography coupled with atomic fluorescence spectrometric detection: Effect of the denaturation on the determination of thiolic proteins
Bramanti E, et al.
Talanta, 63(2), 383-389 (2004)
Triosephosphate isomerase deficiency: new insights into an enigmatic disease
Orosz F, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1792(12), 1168-1174 (2009)
Red blood cell enzymopathies
Hematology, 616-625 (2018)
Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet
Modrego, J., et al.
European Journal of Nutrition, DOI: 10-DOI: 10 (2012)
Zhenlie Huang et al.
Toxicology and applied pharmacology, 263(1), 44-52 (2012-06-12)
1-Bromopropane (1-BP) is neurotoxic in both experimental animals and humans. Previous proteomic analysis of rat hippocampus implicated alteration of protein expression in oxidative stress, suggesting that oxidative stress plays a role in 1-BP-induced neurotoxicity. To understand this role at the
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