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A8811

Sigma-Aldrich

Aldolase from rabbit muscle

ammonium sulfate suspension, 10-20 units/mg protein

Synonym(s):
D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

form

ammonium sulfate suspension

specific activity

10-20 units/mg protein

foreign activity

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%
lactic dehydrogenase ≤0.03%
phosphoglucose isomerase ≤0.6%
pyruvate kinase ≤0.1%
triosephosphate isomerase ≤0.05%

storage temp.

2-8°C

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General description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.[75]

Application

Aldolase from rabbit muscle has been used:
  • in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay
[82]
  • as a standard in the characterization of metabolic enzymes from glaucomatous tissues
  • [17]
  • in fructose 2,6-bisphosphate assay of human cell lines
  • [40]
    Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [46].

    Packaging

    100, 200, 1000 units in poly bottle
    5000 units in glass bottle

    Biochem/physiol Actions

    Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics.[78] It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).[91]
    Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold [46].

    Unit Definition

    One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

    Physical form

    Crystalline suspension in 2.5 M (NH4)2SO4, 0.01 M Tris, pH 7.5, 0.001 M EDTA

    Analysis Note

    Protein determined by biuret.

    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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