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T0198

Sigma-Aldrich

Anti-β-Tubulin antibody, Mouse monoclonal

clone D66, purified from hybridoma cell culture

Synonym(s):
Anti-Tubulin beta-1 chain
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

D66, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

monkey, Oxyrrhis marina (dinoflagellate), rat, mouse, chicken, sea urchin (Lytechinus pictus and Paracentrotus lividus), rabbit, canine, Chlamydomonas reinhardtii, human, bovine, hamster

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

β-Tubulin belongs to the subfamily of tubulins, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β−Tubulin structure is characterized with core of two β -sheets enclosed by a-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (β I (class I), β II (class II), β III (class III), β IVa (class IVa), β IVb (class IVb), β V (class V), and β VI (class VI)).
Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the D66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a sea urchin (Lytechinus pictus) sperm axonemal proteins.

Specificity

Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).

Immunogen

sea urchin (Lytechinus pictus) sperm axonemal proteins.

Application

Can be used in studies involving the inhibition of flagellar motility in permeabilized sperm models.
Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in immunofluorescence assay and enzyme linked immunosorbent assay (ELISA).
Monoclonal Anti-β-tubulin antibody produced in mouse has been used in western blotting and immunocytochemistry.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β- tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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