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SRP6415

Sigma-Aldrich

Cathepsin D human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

CLN10, CPSD, CTSD

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 43.6 kDa
observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P07339

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CTSD(1509)

General description

Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extracellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
This gene is mapped to human chromosome 11p15.5.

Biochem/physiol Actions

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered as an important biomarker for type 2 diabetes dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
Walenbergh SMA, et al.
Scientific reports, 6(1), 38278-38278 (2016)
Cluster analysis of risk factor genetic polymorphisms in Alzheimer?s disease
Randall CN, et al.
Neurochemical Research, 34(1), 23-28 (2009)
Increased Cathepsin D Correlates with Clinical Parameters in Newly Diagnosed Type 2 Diabetes
Liu L, et al.
Disease Markers, 2017(1), 23-28 (2017)
Xiaolai Zhou et al.
Molecular neurodegeneration, 12(1), 62-62 (2017-08-25)
Mutations resulting in progranulin (PGRN) haploinsufficiency cause frontotemporal lobar degeneration with TDP-43-positive inclusions (FTLD-TDP), a devastating neurodegenerative disease. PGRN is localized to the lysosome and important for proper lysosome function. However, the metabolism of PGRN in the lysosome is still
Cathepsin D contributes to the accumulation of advanced glycation end products during photoaging
Xu X, et al.
Journal of Dermatological Science, 90(3), 263-275 (2018)
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