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Sigma-Aldrich

mirPremier® microRNA Isolation Kit

1 sufficient for 10 preparations

Synonym(s):

microRNA Isolation Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

Quality Level

200

usage

sufficient for 10 preparations

technique(s)

DNA extraction: suitable

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General description

Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application

mirPremier® microRNA Isolation Kit has been used to:
  • prepare small RNA
  • enrich small RNAfractions from the human astrocytes for small RNA sequencing and bioinformatics analysis
  • isolate small RNA from Vigna mungo tissues
  • extract miRNAs to detect the expression of miRNA-16

Features and Benefits

  • Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
  • Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Capable of extracting miRNA with high purity with no detectable large RNA.
  • Hazardous organic extractions are not involved.

Legal Information

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

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GHS07

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Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

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MicroRNAs control mRNA fate by compartmentalization based on 3? UTR length in male germ cells
Zhang Y, et al.
Genome Biology, 18(1), 105-105 (2017)

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