Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

SAB4200709

Sigma-Aldrich

Anti-Collagen Type IV antibody, Mouse monoclonal

clone col-94, purified from hybridoma cell culture

Synonym(s):

Anti-col4a1/col4a2

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

col-94, monoclonal

form

buffered aqueous solution

species reactivity

monkey, human

concentration

~1 mg/mL

technique(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: 4-8 μg/mL using formalin-fixed, paraffin-embedded human tongue sections

isotype

IgG1

UniProt accession no.

P02462

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL4A1(1282)

General description

Anti-Collagen Type IV antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the col-94 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with human collagen Type IV. Collagen Type IV (also known as COL4A) is a key component of the basement membranes (BM), which is found at the basal surface of epithelial and endothelial cells and is essential for tissue strength and polarity. Type IV Collagens are encoded by three pairs of paralogous genes (COL4A1 through COL4A6).

Immunogen

human collagen Type IV

Application

Anti-Collagen Type IV antibody, Mouse monoclonal has been used in immunohistochemistry and dot-blotting.

Biochem/physiol Actions

Collagen Type IV represents a physical barrier against cell invasion. Proteolysis/degradation of Collagen Type IV is essential for cell migration and cancer cells invasion through the ECM into the interstitial tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Raghu Kalluri
Nature reviews. Cancer, 3(6), 422-433 (2003-06-05)
In recent years, the basement membrane (BM)--a specialized form of extracellular matrix (ECM)--has been recognized as an important regulator of cell behaviour, rather than just a structural feature of tissues. The BM mediates tissue compartmentalization and sends signals to epithelial
Dynamic interplay between the collagen scaffold and tumor evolution
Egeblad M, et al.
Current Opinion in Cell Biology, 22(5), 697-706 (2010)
Debbie S Kuo et al.
Human molecular genetics, 21(R1), R97-110 (2012-08-24)
Heterotrimers composed of collagen type IV alpha 1 (COL4A1) and alpha 2 (COL4A2) constitute one of the most abundant components of nearly all basement membranes. Accordingly, mutations in COL4A1 or COL4A2 are pleiotropic and contribute to a broad spectrum of
R Grant Rowe et al.
Trends in cell biology, 18(11), 560-574 (2008-10-14)
The basement membrane (BM), a specialized network of extracellular matrix macromolecules, surrounds epithelial, endothelial, muscle, fat and nerve cells. During development, immune surveillance and disease states ranging from cancer to fibrosis, host cells penetrate the BM by engaging tissue-invasive programs
Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry.
S Gay et al.
Methods in enzymology, 145, 148-167 (1987-01-01)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service