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AB748

Sigma-Aldrich

Anti-Collagen Type IV Antibody

Chemicon®, from rabbit

eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

suitability

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

shipped in

dry ice

Gene Information

human ... COL4A1(1282)

Specificity

Antibody reacts with native and heat denatured human type IV collagen. Less than 10% cross reactivity with collagen types I, III, and V, and less than 1% with human collagen II. Antiserum was passed over immobilized human blood plasma proteins, and human collagen types I, III, and V. Human plasma proteins do not interfere with binding to collagen.

Immunogen

Purified human placental collagen type IV

Application

Research Category
Cell Structure
Anti-Collagen Type IV Antibody detects level of Collagen Type IV & has been published & validated for use in ELISA, IH.
Research Sub Category
ECM Proteins
ELISA on human collagen type IV: 1:3,000

Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40, fresh frozen or acetone fixed specimens only.

Dot and slot blots: 1:300.

Not recommended for use in Western blot, or for use on paraffin-embedded tissue sections.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified antibody from cross-absorbed antiserum. Liquid in PBS containing mannitol, dextran and no preservatives.
Format: Purified

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze / thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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