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SAB4200682

Sigma-Aldrich

Anti-Human IgG (Fc specific) antibody, Mouse monoclonal

clone GG-7, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

300

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GG-7, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

ELISA: suitable
immunofluorescence: suitable
indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Human IgG (Fc specific) (mouse IgG1 isotype) is derived from the GG-7 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Monoclonal Anti-Human IgG (Fc specific) antibody has been used in:
  • ELISA
  • Imprint Immunofixation (IIF)
  • Immunofluorometric Assay (IFMA)
  • Hemagglutination (HA)
  • Hemagglutination Inhibition (HAI)
  • Particle Counting Immunoassay (PACIA)
  • detection of cytoplasmic IgG

Biochem/physiol Actions

The crystallizable fragment (Fc) of IgG has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. IgG deficiencies are often associated with various diseases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jefferis R., et al.,
Annales De Biologie Clinique, 52, 57-65 (1994)
Wang L., et al.,
Hybridoma, 1, 293-302 (1982)
Papadea C. and Check IJ.
Critical Reviews in Clinical Laboratory Sciences, 27, 27-58 (1989)
Structure and function of human and murine receptors for IgG
Unkeless J C, et al.
Annual Review of Immunology, 6(1), 251-281 (1988)
C B Reimer et al.
Hybridoma, 3(3), 263-275 (1984-01-01)
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2

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