I9135

Anti-Human IgG (Fc specific) antibody produced in rabbit

whole antiserum

• Synonym(s): Human Antiserum, Rabbit Anti Human IgG - Anti-Human IgG (Fc specific) antibody produced in rabbit, Rabbit Anti Human Igg, Rabbit Antiserum
• MDL number: MFCD00162436
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:100,000; quantitative precipitin assay: 1.5 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that are associated by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

Application

Anti-Human IgG (Fc specific) antibody produced in rabbit was used in immunoblotting and immunocytochemistry.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a pivotal role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be linked to allergy and has the longest serum half life.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Preparation Note

treated to remove lipoproteins

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable