SAB4200597

Monoclonal Anti-FMR1 antibody produced in mouse

clone FMR2.5, hybridoma cell culture supernatant

• Synonym(s): FMRP, FRAXA, POF, POF1, fragile X mental retardation 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FMR2.5, monoclonal
• form: buffered aqueous solution
• mol wt: ~71 kDa
• species reactivity: rat, human, mouse, hamster
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 2.0-5.0 μg/test using HeLa cells; immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts.; immunocytochemistry: suitable; immunofluorescence: 5-10 μg/mL using HepG2 cells.
• isotype: IgG1
• UniProt accession no.: Q06787
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FMR1(2332) ; mouse ... Fmr1(14265) ; rat ... Fmr1(24948)

Description

General description

Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.

Specificity

Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1

Application

Monoclonal Anti-FMR1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

Biochem/physiol Actions

Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable