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S4944

Sigma-Aldrich

Monoclonal Anti-Sonic Hedgehog (N-terminal) antibody produced in rat

clone 171018, purified immunoglobulin, lyophilized powder

Synonym(s):
Anti-Shh
MDL number:
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

171018, monoclonal

form

lyophilized powder

species reactivity

mouse, human

technique(s)

capture ELISA: suitable
immunohistochemistry (frozen sections): 25 μg/mL using mouse embryo
neutralization: suitable
western blot: 1-2 μg/test

isotype

IgG2a

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... SHH(6469)
mouse ... Shh(20423)

Immunogen

purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).

Biochem/physiol Actions

Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, gamma catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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SDS

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