S4944

Monoclonal Anti-Sonic Hedgehog (N-terminal) antibody produced in rat

clone 171018, purified immunoglobulin, lyophilized powder

• Synonym(s): Anti-Shh
• MDL number: MFCD06798717
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 171018, monoclonal
• form: lyophilized powder
• species reactivity: mouse, human
• technique(s): capture ELISA: suitable; immunohistochemistry (frozen sections): 25 μg/mL using mouse embryo; neutralization: suitable; western blot: 1-2 μg/test
• isotype: IgG2a
• UniProt accession no.: Q15465
• storage temp.: −20°C
• Gene Information: human ... SHH(6469) ; mouse ... Shh(20423)

Description

Immunogen

purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).

Biochem/physiol Actions

Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, gamma catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves