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S4760

Sigma-Aldrich

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

elastase substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide, N-Succinyl-tri-L-alanine 4-nitroanilide

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About This Item

Empirical Formula (Hill Notation):
C19H25N5O8
CAS Number:
52299-14-6
Molecular Weight:
451.43
Beilstein/REAXYS Number:
2928351
EC Number:
257-823-5
MDL number:
MFCD00036774
UNSPSC Code:
12352204
PubChem Substance ID:
24899617
NACRES:
NA.32

product name

N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate

Quality Level

200

assay

≥98% (HPLC)

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

SMILES string

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

InChI key

GVUGADOWXGKRAE-SRVKXCTJSA-N

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General description

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is a chromogenic substrate for chymotrypsin-like serine protease, pancreatic elastase and serine endopeptidase.

Application

N-Succinyl-Ala-Ala-Ala-p-nitroanilide has been used as a substrate
  • for screening elastase activity in lyophilized earthworm fibrinolytic enzymes
  • for quantifying elastase activity in sheep milk
  • in screening honeybee inhibitors in serine protease inhibition assay

Substrates

Elastase substrate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Role of endogenous enzymes in proteolysis of sheep milk
Albenzio M, et al.
Journal of Dairy Science, 92(1), 79-86 (2009)
Ning Xu et al.
Frontiers in microbiology, 8, 1834-1834 (2017-10-07)
During parasite infection, serine protease inhibitors secreted by parasites play important roles in suppressing host defenses. However, the mechanism of immune regulation is unclear. In this study, a serpin gene from
Ulvi Kahraman Gürsoy et al.
Pathogens (Basel, Switzerland), 9(9) (2020-09-19)
Aim was to profile salivary total protease, Porphyromonas gingivalis gingipain, and neutrophil elastase activities in relation to the resolution of periodontal inflammation, salivary macrophage-derived chemokine (MDC), and macrophage inflammatory protein-1α concentrations. Nonsurgical periodontal treatment was performed in 24 periodontitis patients
V J Uitto et al.
Journal of clinical periodontology, 23(1), 30-37 (1996-01-01)
The study was designed to find out whether oral elastase activity could be used as a simple biochemical indicator of periodontal health. Both stimulated whole saliva and water rinse samples were collected from subjects with different degrees of adult periodontitis
SEP-1-a subtilisin-like serine endopeptidase from germinated seeds of Hordeum vulgare L. cv. Morex
Fontanini D and Jones BL
Planta, 215(6), 885-893 (2002)
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Articles

Elastase

Enzyme Explorer Product Application Index for Elastase. Leukocyte elastase is a 29KDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds.

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