Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

Elastase from hog pancreas, Pancreatopeptidase E
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

biological source


Quality Level


lyophilized powder

specific activity

≥4 units/mg protein

mol wt

25.9 kDa


pkg of 1 μmol


cell culture | mammalian: suitable




suitable for

foreign activity

Trypsin ≤50 BAEE units/mg protein

shipped in

dry ice

storage temp.


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General description

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.


Elastase from porcine pancreas is used for the following applications:
  • Cell culture techniques
  • ELISA techniques
  • Used in assay for elastase inhibitory activity


1, 5 mg in glass bottle
20 mg in poly bottle
Package size based on protein content

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Unit Definition

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.


Exclamation markHealth hazard

Signal Word


Hazard Statements


Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system


11 - Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
The presence of antibodies against pancreatic juice (PAB) in patients with Crohn's disease has recently been reported. In our study sera from 273 patients with inflammatory bowel disease (222 with Crohn's disease, 51 with ulcerative colitis) have been examined for...
Yoshiyuki Okumura et al.
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid...
Adrien Lupieri et al.
Cardiovascular research, 116(2), 438-449 (2019-05-21)
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune...
P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as...
Denisa Beňová-Liszeková et al.
Microscopy research and technique, 82(7), 1145-1156 (2019-03-27)
Although scanning electron microscopy (SEM) has been broadly used for the examination of fixed whole insects or their hard exoskeleton-derived structures, including model organisms such as Drosophila, the routine use of SEM to evaluate vulnerable soft internal organs and tissues...
Enzymatic Assay of Elastase (EC, a continuous spectrophotometric rate determination.
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