Rat Tumor Necrosis Factor α ELISA Kit

for cell and tissue lysates


species reactivity



kit of 96 wells (12 strips x 8 wells)


ELISA: suitable
capture ELISA: suitable


sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

detection method


shipped in

wet ice

storage temp.


Gene Information

rat ... Tnf(24835)

General description

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate.


Recombinant Rat TNF-α


For research use only. Not for use in diagnostic procedures.
Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate.

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABLYSIS12X Cell Lysis Buffer (Item J)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)



Signal Word


Hazard Statements


Met. Corr. 1


8B - Non-combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Effects of kefir on ischemia-reperfusion injury.
Yener AU
European Review for Medical and Pharmacological Sciences, 19(5), 887-896 (2015)
Tumor necrosis factor-alpha: a possible priming agent for the polymorphonuclear leukocyte-reduced nicotinamide-adenine dinucleotide phosphate oxidase in hypertension.
Mazor R
Hypertension, 55(2), 353-362 (2010)
R A Ajala-Lawal et al.
Archives of physiology and biochemistry, 126(2), 107-115 (2018-10-06)
This study investigated the influence of betulinic acid on high-fructose diet-induced metabolic syndrome in rats. Oral administration of betulinic acid significantly reversed high-fructose diet-mediated increase in body mass index and blood glucose. Furthermore, betulinic acid restored high-fructose diet-mediated alterations in...
Diosmin Modulates the NF-kB Signal Transduction Pathways and Downregulation of Various Oxidative Stress Markers in Alloxan-Induced Diabetic Nephropathy.
Ahmed S
Inflammation, 39(5), 1783-1797 (2016)
Tumor necrosis factor-alpha. A mediator of focal ischemic brain injury.
Barone FC
Stroke, 28(6), 1233-1244 (1997)

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