R5125

Ribonuclease A from bovine pancreas

Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

• Synonym(s): Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
• CAS Number: 9001-99-4
• Enzyme Commission number: 3.1.27.5 (BRENDA, IUBMB)
• EC Number: 232-646-6
• MDL number: MFCD00132181
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine pancreas
• type: Type III-A
• assay: ≥85% RNase A basis (SDS-PAGE)
• form: lyophilized powder
• specific activity: 85-140 Kunitz units/mg protein
• mol wt: ~13,700
• technique(s): cell based assay: suitable
• impurities: salt, essentially free
• suitability: suitable for molecular biology
• application(s): diagnostic assay manufacturing
• foreign activity: protease, essentially free
• storage temp.: −20°C
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• InChI key: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Application

• RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
• RNase A is also used in RNA sequence analysis and protection assays.
• RNase A has been used as a tool for computer-aided drug design.
• RNase A supports the analysis of RNA sequences.
• RNase A hydrolyze RNA contained in protein samples.
• Purification of DNA is supported by RNase A.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess nickel-dependent oxidative cross-linking of a protein. Ribonuclease A from bovine pancreas has also been used in a study to investigate equilibrium constants for nonspecific binding of proteins to DNA.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Preparation Note

Salt fractionated and chromatographically purified.

Analysis Note

Protein determined by E.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)