All Photos(4)

D8764

Sigma-Aldrich

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym(s):
Deoxyribonucleate 3′-oligonucleotido-hydrolase, DNase II
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

Quality Level

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

38 kDa

foreign activity

RNase ≤0.1%

storage temp.

−20°C

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Application

Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples
  • to digest retinal explants for single-cell suspension preparation
  • for isolation of stromal cells from human colon tissue
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Packaging

30000, 150000, 300000 units in poly bottle

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Technical Information

More documents

Quotes and Ordering

STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
G BERNARDI et al.
Biochemistry, 3, 1419-1426 (1964-10-01)
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
Candace L Minchew et al.
Molecules (Basel, Switzerland), 16(6), 4599-4614 (2011-06-07)
In apoptosis, the initial self-driven suicide phase generates cellular corpses which are digested in the phagolysosomes of professional and amateur phagocytes during the subsequent waste-management phase. This ensures the complete elimination of the genetic material which often contains pathological, viral

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