All Photos(2)

D4138

Sigma-Aldrich

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(s):
Deoxyribonucleate 3′-oligonucleotido-hydrolase, DNase II
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

Quality Level

type

Type IV

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Application

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).

Packaging

20000, 80000 units in poly bottle

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM

Physical form

Contains sodium chloride

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Technical Information

More documents

Quotes and Ordering

C C Wang et al.
The Journal of biological chemistry, 273(27), 17192-17198 (1998-06-27)
Porcine spleen DNase II, a lysosomal acid hydrolase, is a noncovalently linked alpha.beta heterodimer (Liao, T.-H. (1985) J. Biol. Chem. 260, 10708-10713). The alpha subunit, after disulfide cleavage, yields two chains, alpha1 and alpha2. The complete amino acid sequences of
F Chianini et al.
Veterinary immunology and immunopathology, 82(3-4), 245-255 (2001-10-06)
The distribution of different cells of the immune system has been studied in formalin-fixed paraffin-embedded tissues from conventionally reared healthy pigs, using immunohistological techniques. The samples collected were: lungs, tonsils, lymph nodes (mediastinal, mesenteric, inguinal and submandibular), pancreas, spleen, liver
U Plöckinger et al.
European journal of endocrinology, 166(2), 223-234 (2011-11-09)
Somatostatin analogues (SSA) reduce autonomous GH secretion by activating somatostatin receptors (sst) 2 and 5 in 50-60% of acromegalic patients. However, by inhibiting insulin secretion these SSA reduce glucose tolerance. DG3173 is a novel SSA with additional binding to sst4
Yoko Kato-Toma et al.
The Biochemical journal, 371(Pt 1), 175-181 (2003-01-07)
13C-NMR spectroscopy was used to estimate the p K a values for the Tyr(150) (Y150) residue in wild-type and mutant class C beta-lactamases. The tyrosine residues of the wild-type and mutant lactamases were replaced with (13)C-labelled L-tyrosine ([ phenol -4-(13)C]tyrosine)
Lars Holmgren
Biochemical and biophysical research communications, 396(1), 147-151 (2010-05-25)
Horizontal or lateral gene transfer is an effective mechanism for the exchange of genetic information in bacteria allowing bacterial diversification and facilitating adaptation to new environments. Recent data demonstrate that DNA may also be transferred between somatic cells via the

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