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Key Documents

R2283

Sigma-Aldrich

Pepsin Reagent, Ready to Use, Antigen Retriever

suitable for immunohistochemistry

Synonym(s):

pepsin

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

biological source

Porcine stomach

Quality Level

application(s)

hematology
histology

storage temp.

2-8°C

Application

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC).
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Codistribution of collagen type IV and laminin in liver fibrosis of elderly cadavers: immunohistochemical marker of perisinusoidal basement membrane formation.
Mak Ki M, et al.
The Anatomical Record, 296(6)A, 953-964 (2013)
The effect of low doses of cadmium and zinc chloride on the Blood-Testis Barrier of Sprague Dawley rats.
Redondo A, et al.
arXiv (2012)
Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes.
Rwigemera A, et al.
Epigenetics & Chromatin, 10(1), 19-19 (2017)

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