P9541
for molecular biology
≥99.0%
powder
770 °C (lit.)
1.98 g/mL at 25 °C (lit.)
Fe: ≤3 ppm
heavy metals (as Pb): ≤5 ppm
DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
[Cl-].[K+]
1S/ClH.K/h1H;/q;+1/p-1
WCUXLLCKKVVCTQ-UHFFFAOYSA-M
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13 - Non Combustible Solids
WGK 1
Not applicable
Not applicable
dust mask type N95 (US), Eyeshields, Gloves
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TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition
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