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P7582

Sigma-Aldrich

Amino-terminal FLAG-BAP Fusion Protein

MDL number:
NACRES:
NA.32

form

liquid

Quality Level

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.
Amino-terminal FLAG-BAP Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.

Application

Amino-terminal FLAG-BAP Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.
Browse additional application references in our FLAG® Literature portal.

Other Notes

Control protein

Physical form

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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