Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.
Mouse anti-(p53) antibody reacts specifically with (p53) if human.
recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.
Anti-p53 antibody, Mouse monoclonal has been used in:
- semi-dry western blotting
- enzyme-linked immunosorbent assay (ELISA)
- flow cytometry
- chromatin immunoprecipitation (ChIP) assay
The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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