MilliporeSigma
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P5813

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone BP53-12, ascites fluid

Synonym(s):
Anti-TRP53, Anti-Transformation-related protein 53
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BP53-12, monoclonal

mol wt

antigen 53 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TP53(7157)

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General description

Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor.[52][53] TP53 gene is mapped to human chromosome 17p13.1.[96] The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells.

Immunogen

recombinant human wild-type p53 protein.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • western blotting
[54]
  • immunohistochemistry
  • [55][56][57]
  • immunocytochemistry
  • [58]
  • enzyme linked immunosorbent assay (ELISA)

  • Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

    Biochem/physiol Actions

    The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage.[97] Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer.[49] They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy.[51] Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins.

    Disclaimer

    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    Storage Class Code

    10 - Combustible liquids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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    SDS

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