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NPT01

Sigma-Aldrich

NeuroPorter Transfection Kit

Lipid formulation for nucleic acid transfections in neuronal and glial cells

NACRES:
NA.25

Quality Level

grade

for molecular biology

form

dried film

usage

 kit sufficient for 75-200 transfections

availability

available only in USA, Canada and EU

technique(s)

transfection: suitable

storage temp.

2-8°C

General description

Neuroporter Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.

Application

Suitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 μl Neuroporter Transfection Reagent and 6-8 μg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter Transfection Kit:

  • C6 glioma (human)
  • Cortical neurons (rat primary)
  • Dorsal Root Ganglion (DRG) cells (rat)
  • NT2 neurons(human precursor cells)
  • NT neurons (human differentiated cells)
  • Subventricular Zone (SVZ) cells (mouse)
  • White matter cells (mouse)

Features and Benefits


  • Optimized for primary neurons, glial cells, and cultured neural cell lines
  • Very low toxicity with no neuro-degeneration or dendrite withdrawal
  • Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines
  • Fast and easy to use compared to other methods
  • Compatible with both serum and serum-free transfection protocols

Components

1 vial Neuroporter Transfection Reagent, dried lipid film (T2823)
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941

Caution

Do not freeze.

Principle

A stable, non-covalent complex is formed when the Neuroporter Transfection Reagent is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

NeuroPorter is a trademark of Gene Therapy Systems, Inc.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Articles

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Related Content

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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