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L3037

Escort^™ III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

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NACRES:

NA.25

Quality Level

200

grade

for molecular biology

form

liquid (aqueous solution)

usage

mL sufficient for 250-1000 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

General description

Escort^™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort^™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort^™ III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

Features and Benefits

Suitable for stable and transient transfection
Optimized for a wide variety of primary cells
Low toxicity
Compatible with both serum and serum-free transfection protocols
Ideal for PC-12 cells

Components

Escort^™ III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort^™ III is confidential.

Caution

Do not freeze.

Principle

A stable complex is formed when Escort^™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

Lipid Transfection Optimization Protocol

Expert guidance on the best way to optimize your transfection experiments. Optimized protocols result in highest efficiency transfections.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort^™ III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

Properties