N1142

Neomycin

solution, suitable for cell culture, BioReagent

• Synonym(s): Antibiotic 10676, Neomycin B
• UNSPSC Code: 12352207
• PubChem Substance ID: 24897464
• NACRES: NA.76

Properties

• product name: Neomycin solution, with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture
• biological source: Streptomyces fradiae
• Quality Level: 200
• sterility: 0.1 μm filtered
• product line: BioReagent
• form: liquid
• potency: 8-12 mg per mL
• packaging: pkg of 20ML
• concentration: 10 mg/mL in 0.9% NaCl (neomycin)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: clear
• UniProt accession no.: O14746
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]1O
• Gene Information: human ... TERT(7015)

Description

General description

Chemical structure: aminoglycoside

Application

Neomycin solution has been used:

• as a supplement of autoclaved acidified water for bone marrow transplantation
• for the selection of stable clones
• as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures

Biochem/physiol Actions

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca²⁺ channels without affecting the Na⁺/Ca²⁺ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria

Caution

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter