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Key Documents

MAK501

Sigma-Aldrich

Glucose Oxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Glucose Oxidase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

application(s)

food and beverages
pharmaceutical

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes

shipped in

wet ice

storage temp.

−20°C

General description

Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients.

Features and Benefits

  • Linear detection range: 0.02 to 10 U/L glucose oxidase for the colorimetric assay and 0.002 to 1.5 U/L for the fluorometric assay.

Suitability

The kit is suitable for determining glucose oxidase activity in cell lysate, culture medium and other biological samples, as well as for studying the effects of drugs on glucose metabolism.

Principle

The Glucose Oxidase Assay Kit uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to glucose oxidase activity in the sample.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

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