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M9004

Sigma-Aldrich

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonym(s):
L-Malate:NAD+ oxidoreductase, MDH
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bovine heart

Quality Level

form

ammonium sulfate suspension

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

mol wt

35 kDa

impurities

≤0.01% Glutamic-Oxalacetic Transaminase
≤0.01% Glutamic-Pyruvic Transaminase

storage temp.

2-8°C

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General description

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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