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Malic Dehydrogenase from porcine heart

≥600 units/mg protein (biuret), ammonium sulfate suspension

L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
Enzyme Commission number:
EC Number:
MDL number:


ammonium sulfate suspension

Quality Level

specific activity

≥600 units/mg protein (biuret)

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

storage temp.


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General description

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.[84]
Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity. [08]
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.


Malic Dehydrogenase from porcine heart has been used:
  • in qualitative protein binding measurements
  • to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
  • [86]
  • to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase
  • [67]
    Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. [39] It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid. [55]

    Biochem/physiol Actions

    Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate.[84]

    Unit Definition

    One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

    Physical form

    Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0

    Storage Class Code

    13 - Non Combustible Solids


    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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