MilliporeSigma
All Photos(1)

M1567

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

≥600 units/mg protein (biuret), ammonium sulfate suspension

Synonym(s):
L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

specific activity

≥600 units/mg protein (biuret)

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.[84]
Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity. [08]
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic Dehydrogenase from porcine heart has been used:
  • in qualitative protein binding measurements
[85]
  • to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
  • [86]
  • to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase
  • [67]
    Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. [39] It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid. [55]

    Biochem/physiol Actions

    Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate.[84]

    Unit Definition

    One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

    Physical form

    Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0

    Storage Class Code

    13 - Non Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

    More Documents

    SDS

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service