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M3024

Sigma-Aldrich

Minimum Essential Medium Eagle

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

Synonym(s):
MEM, EMEM
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
NaHCO3: no
Earle’s salts (5% CO2): yes
L-glutamine: no
phenol red: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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Application

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Quantity

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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