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M0643

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
MEM, EMEM
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
Earle’s salts (5% CO2): yes
L-glutamine: yes
HEPES: no
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.
It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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