M1018

Minimum Essential Medium Eagle

With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): MEM
• NACRES: NA.75

Properties

• form: powder
• technique(s): cell culture | mammalian: suitable
• components: Hanks’ salts (2% CO₂): yes; sodium pyruvate: no; NaHCO₃: no; L-glutamine: yes; phenol red: yes; HEPES: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Quantity

Formulated to contain 10.7 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable