M0445

Anti-MDMX antibody, Mouse monoclonal

clone MDMX-82, purified from hybridoma cell culture

• Synonym(s): Anti-HDMX, Anti-MDM4, Anti-p53 Binding Protein
• MDL number: MFCD00282354
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MDMX-82, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of HEK 293T cells transfected with human MDMX
• isotype: IgG1
• UniProt accession no.: O15151
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MDM4(4194) ; mouse ... Mdm4(17248) ; rat ... Mdm4(304798)

Description

General description

Double minute 4 protein or MDMX is a nuclear protein. The gene encoding it is localized on human chromosome 1q32.1.

Monoclonal Anti-MDMDX (mouse IgG1 isotype) is derived from the hybridoma MDMX-82 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. MDMX belongs to the MDM family. The MDMX/ mouse double minute 4 (MDM4) gene encode a 490 amino acid protein. MDMX comprises a putative nuclear localization signal, a really interesting new gene (RING) finger domain in the C terminal and a p53-binding domain in the N terminal. MDMX has significant structural similarity to MDM2 E3 ubiquitin ligase.

Specificity

Monoclonal Anti-MDMX recognizes human MDMX.

Application

Monoclonal Anti-MDMX antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot (approx. 80 kDa), microarray, and ELISA. The product may also be used for western blot and for assessing ectopic expression of MDMX (using a conc. of 1-2 μg/mL in total cell extract of HEK 293T cells transfected with human MDMX).

Biochem/physiol Actions

MDMX is a structural homolog of MDM2 and inhibits the tumor suppressor, p53. MDMX also prevents MDM2-mediated degradation of p53, and subsequently regulates cellular levels of p53. MDMX also modulates cell growth and survival. Thus, MDMX can be useful as a therapeutic target in cancers, especially in tumors where native p53 functions can be restored . Monoclonal Anti-MDMX antibody is specific for human MDMX.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)