L3410
Anti-Leptin antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Leptin Antibody, Leptin Antibody - Anti-Leptin antibody produced in rabbit, Anti-OB
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, human
technique(s)
indirect ELISA: 1:1,000 using human or mouse recombinant leptin
microarray: suitable
western blot: 1:5,000 using human recombinant leptin
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LEP(3952)
mouse ... Lep(16846)
Related Categories
General description
The gene for leptin (LEP) is mapped to the human chromosome 7q32.1. The encoded protein is a non-glycosylated polypeptide, primarily produced and secreted by the mature adipocytes. Leptin is expressed in white adipose tissue, fetal tissues, skeletal muscle, chondrocytes, gastric chief cells, the placenta, ovary, and many immune cells. It is absent or expressed at extremely low levels in other adult tissues. Human leptin is 85% identical to mouse leptin and 84% identical to rat leptin, while mouse and rat exhibit 96% identity in their leptin molecules.
Specificity
Anti-Leptin reacts specifically with leptin.
Immunogen
synthetic peptide corresponds to the amino terminal (amino acid residues 22-40) of human/mouse leptin.
Application
Anti-Leptin antibody produced in rabbit has been used in:
- western blotting
- immunohistochemistry
- immunocytochemistry
- enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Leptin is a circulating cytokine that induces pleiotropic effects on nutrient intake, thermogenesis, metabolism, reproduction and hematopoiesis. Leptin is hypothesized to be a “satiety factor” because the absence of this factor is associated with hyperphagia and obesity in ob/ob mice (homozygous for the obese spontaneous mutation). It appears that leptin is involved in appetite regulation since leptin injection into ob/ob mice reduces their food intake and ultimately their body weight. However, studies have demonstrated that its effects must be more complicated than simple appetite suppression.
Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism and gastric mobility. The pathways that mediate the effect of leptin are Janus kinase (JAK)/signal transducer and activator of transcription (STAT), phosphoinositide 3-kinase (PI3K) and c-Jun N-terminal kinase (JNK).
Physical form
Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
The antibody is affinity isolated on peptide-agarose.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Mónica Z Jenks et al.
Scientific reports, 7(1), 8232-8232 (2017-08-16)
Leptin is an adipokine produced by fat cells that regulates food consumption and metabolic activity. Sexual dimorphism in leptin and fat stores have been observed in humans and rodents with females having more leptin and greater levels of subcutaneous fat
Angela Katherine Odle et al.
Endocrinology, 157(4), 1443-1456 (2016-02-10)
Leptin regulates food intake and energy expenditure (EE) and is produced in adipocytes, the pituitary, and several other tissues. Animals that are leptin or leptin receptor deficient have major metabolic complications, including obesity. This study tests the hypothesis that the
Sandra Barbosa-da-Silva et al.
PloS one, 7(7), e39837-e39837 (2012-08-01)
Obesity is associated with low-grade chronic inflammation attributed to dysregulated production, release of cytokines and adipokines and to dysregulated glucose-insulin homeostasis and dyslipidemia. Nutritional interventions such as dieting are often accompanied by repeated bouts of weight loss and regain, a
Kate Sullivan et al.
Human molecular genetics, 23(5), 1250-1259 (2013-10-29)
There is emerging evidence for reduced muscle function in children with neurofibromatosis type 1 (NF1). We have examined three murine models featuring NF1 deficiency in muscle to study the effect on muscle function as well as any underlying pathophysiology. The
Weihong Pan et al.
Annals of the New York Academy of Sciences, 1264, 64-71 (2012-04-26)
Leptin, an adipocyte-derived cytokine, crosses the blood-brain barrier to act on many regions of the central nervous system (CNS). It participates in the regulation of energy balance, inflammatory processes, immune regulation, synaptic formation, memory condensation, and neurotrophic activities. This review
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service