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KMM-101NV

Sigma-Aldrich

KOD One PCR Master Mix

Ready-to-use 2X PCR master mix with a hot-start modified KOD DNA polymerase optimized for fast and convenient high fidelity PCR

Synonym(s):
Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One PCR Ready Mix

Quality Level

biological source

Escherichia coli (carrying the cloned UKOD DNA polymerase gene)

form

liquid

usage

sufficient for 25 reactions
sufficient for 500 reactions

feature

Fast PCR
High Fidelity PCR
dNTPs included
hotstart

technique(s)

PCR: suitable

input

crude DNA

shipped in

dry ice

storage temp.

−20°C

General description

KOD One PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.

This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.

Application

  • Genomic DNA amplification
  • cDNA amplification
  • Direct PCR
  • Colony PCR
  • Amplification of NGS libraries
  • Site direct gene mutation

Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA

Features and Benefits

  • Fast: Amplifies the targets using the following very short conditions:
o =1 kb: 1 sec
o 1~ 10 kb: 5 sec/ kb
o 10 kb~: 10 sec/ kb
  • Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.
  • High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
  • High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
  • Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
  • High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)

Packaging

KMM-101NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.

Other Notes

For R&D use only. Not for drug, household, or other uses.

Legal Information

*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Protocols

Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance

Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.

Related Content

KOD One™ PCR Master Mix for Ultra-Fast PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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