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KCQS04

Sigma-Aldrich

KiCqStart® Probe qPCR ReadyMix™

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

Synonym(s):

Kicqstart® Probe qPCR ReadyMix™

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

form

liquid

usage

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

feature

dNTPs included
hotstart

storage condition

protect from light

technique(s)

qPCR: suitable

color

light blue

input

purified DNA

compatibility

Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex
Eppendorf® Mastercycler ep realplex2 S
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480

detection method

probe-based

shipped in

dry ice

storage temp.

−20°C

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General description

KiCqStart® Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

Features and Benefits

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Disruption of KIF3A in patient-derived glioblastoma cells: effects on ciliogenesis, hedgehog sensitivity, and tumorigenesis
Lan B
Oncotarget (2016)

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Probe based QPCR utilizes a fluorescent–labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

PCR/qPCR Data Analysis

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PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Quantitative PCR Basics

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