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I4753

Sigma-Aldrich

Invertase from Candida utilis

Grade X, ≥300 units/mg solid

Synonym(s):

β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Grade X

Quality Level

form

solid

specific activity

≥300 units/mg solid

mol wt

~260 kDa

foreign activity

α-galactosidase (melibiase), essentially free

storage temp.

−20°C

General description

Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.

Application

Invertase from Candida utilis has been used:
  • as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
  • to determine the sucrose content in ethanolic extract of leaf tissues
  • to determine the total non-structural carbohydrates (TNC) in root tissues

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

Biochem/physiol Actions

Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.

Physical properties

Michaelis constant : 1.5x10 -2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70oC
pH Stability : pH 4.0 - 6.0 (50oC, 10min)
Thermal stability : below 60oC (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6)

Unit Definition

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

Preparation Note

Chromatographically purified

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Interactive effects of elevated carbon dioxide and growth temperature on photosynthesis in cotton leaves
Reddy AR, et al.
Plant Growth Regulation, 26(1), 33-40 (1998)
Purification and characterization of an invertase from Candida utilis: comparison with natural and recombinant yeast invertases
Chavez FP, et al.
Journal of Biotechnology, 53(1), 67-74 (1997)
Winter foot and equipment traffic impacts on a `L93?creeping bentgrass putting green
Baldwin CM, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 43(3), 922-926 (2008)
Invertase and its applications-a brief review
Kulshrestha S, et al.
Journal of Pharmacy Research, 7(9), 792-797 (2013)
Morphological and physiological changes in Streptomyces lividans induced by different yeasts
Santamaria R, et al.
Archives of Microbiology, 177(3), 259-266 (2002)

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