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Key Documents

HCLBRST

Sigma-Aldrich

Breast Analyte Control

Cell line controls for immunohistochemistry and in situ hybridization

Synonym(s):

ER/PGR/HER2 Analyte Control

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.47

biological source

human cell culture

packaging

pkg of 1 ea cell microarray block (HCL015)
pkg of 2 slides (HCL013)
pkg of 5 slides (HCL014)

manufacturer/tradename

Histocyte

storage temp.

2-8°C

Related Categories

General description

The Breast Analyte Control has two cell line cores which demonstrate positive and negative expression of ER, PGR, and HER2.
HistoCyte Laboratories control slides and blocks are made from cultured human cell lines to provide the required range of expression of a specific biomarker. The cell lines are processed using proprietary methods that allow for the creation of a high density embedded cell block with cores that retain their cellular morphology. Available as pre-cut unstained slides or as cell microarray blocks for sectioning.

Application

Ideal for use as a same slide control for immunohistochemistry (IHC) and in situ hybridization (ISH).
The Breast Analyte Control histology slides have two cell line cores providing positive and negative expression of ER, PGR, and HER2. Analyte controls are placed at one end of slide, allowing sample application on same slide for valid comparison.
The Breast Analyte Control cell microarray block contains two cell line cores that are 2 mm in diameter and between 3.0-3.5 mm in length. The cell microarray block may yield over 300 sections depending on thickness.

Preparation Note

Cell microarray blocks are fixed in 10% neutral buffered formalin and embedded in paraffin wax. Pre-cut unstained slides are prepared from 4 μm thick sections, mounted on positively charged slides, and dried at 37 °C overnight.

Other Notes

For certificates of analysis, contact info@histocyte.com.
Breast Analyte Control brochure is available from HistoCyte Laboratories.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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