MilliporeSigma
All Photos(1)

H8509

Sigma-Aldrich

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Type V, lyophilized powder, 250-750 units/mg protein

All Photos(1)
Synonym(s):
(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH
CAS Number:
9028-38-0
Enzyme Commission number:
1.1.1.30 (BRENDA, IUBMB)
EC Number:
232-830-6
MDL number:
MFCD00130633
NACRES:
NA.54

type

Type V

Quality Level

200

form

lyophilized powder

specific activity

250-750 units/mg protein

purified by

chromatography

foreign activity

lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

1 of 4

This Item
H9408F8649H1506
Sigma-Aldrich

Sigma-Aldrich

H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei lyophilized powder, ≥200 units/mg protein

Sigma-Aldrich

H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

Formate Dehydrogenase from Candida boidinii lyophilized powder, 5.0-15.0 units/mg protein

Sigma-Aldrich

F8649

Formate Dehydrogenase from Candida boidinii

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni ≥15 units/mg protein

Sigma-Aldrich

H1506

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized solid

specific activity

250-750 units/mg protein

specific activity

≥200 units/mg protein

specific activity

5.0-15.0 units/mg protein

specific activity

≥15 units/mg protein

purified by

chromatography

purified by

chromatography

purified by

-

purified by

chromatography

foreign activity

lactate dehydrogenase ≤0.05%, malate dehydrogenase ≤0.1%

foreign activity

lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%

foreign activity

-

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Unit Definition

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service