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H8280

Sigma-Aldrich

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

matrix fast-flow highly cross-linked 4% beaded agarose

Synonym(s):

N-Hydroxysuccinimidyl-Agarose

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About This Item

MDL number:
MFCD00164950
UNSPSC Code:
23151817
NACRES:
NA.56

form

isopropanol suspension

Quality Level

200

extent of labeling

16-23 μmol per mL

technique(s)

protein array: suitable

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix active group

N-hydroxysuccinimide active ester

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

storage temp.

2-8°C

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General description

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

  • Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation, which gives a high degree of substitution of the selected ligand
  • NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs

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