MilliporeSigma
All Photos(1)

GW22473C

Sigma-Aldrich

Anti-PRKCQ antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

NACRES:
NA.41

biological source

chicken

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, human, mouse

manufacturer/tradename

Genway 15-288-22473C

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKCQ(5588)

General description

PRKCQ (protein kinase C θ) is majorly expressed in T-cells, and is a member of the PKC protein family. It belongs to the subfamily of proteins which are induced by diacylglycerol (DAG), rather than Ca2+. It is the only PKC member that is localized to the immunological synapse.

Immunogen

Immunogen Sequence: GI # 5453976, sequence 610-673
Recombinant protein kinase C, theta

Application

Anti-PRKCQ antibody produced in chicken is suitbale for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Protein kinase C θ (PKC-θ) promotes epithelial to mesenchymal transition (EMT) by acting as a critical chromatin-anchored switch for inducible genes. Chromatinized PKC-θ exists as an active transcription complex and is essential to establish a permissive chromatin state at signature EMT genes. It plays a central role in TCR-induced IL-2 production and T-cell proliferation. PKCθ activity is regulated by the intracellular redox state. It is the only T cell-expressed PKC that localizes selectively to the center of the immunological synapse (IS) following conventional T cell antigen stimulation. This unique localization is required for PKCθ-mediated downstream signaling. These attributes of PKCθ makes it an attractive target for catalytic or allosteric inhibitors. PKCθ may be associated with type 2 diabetis.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service