MilliporeSigma
  • Home
  • Search Results
  • PKCθ regulates T cell motility via ezrin-radixin-moesin localization to the uropod.

PKCθ regulates T cell motility via ezrin-radixin-moesin localization to the uropod.

PloS one (2013-11-20)
Judy L Cannon, Francois Asperti-Boursin, Kenneth A Letendre, Ivy K Brown, Katy E Korzekwa, Kelly M Blaine, Sreenivasa R Oruganti, Anne I Sperling, Melanie E Moses
ABSTRACT

Cell motility is a fundamental process crucial for function in many cell types, including T cells. T cell motility is critical for T cell-mediated immune responses, including initiation, activation, and effector function. While many extracellular receptors and cytoskeletal regulators have been shown to control T cell migration, relatively few signaling mediators have been identified that can modulate T cell motility. In this study, we find a previously unknown role for PKCθ in regulating T cell migration to lymph nodes. PKCθ localizes to the migrating T cell uropod and regulates localization of the MTOC, CD43 and ERM proteins to the uropod. Furthermore, PKCθ-deficient T cells are less responsive to chemokine induced migration and are defective in migration to lymph nodes. Our results reveal a novel role for PKCθ in regulating T cell migration and demonstrate that PKCθ signals downstream of CCR7 to regulate protein localization and uropod formation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-PRKCQ antibody produced in chicken, affinity isolated antibody, buffered aqueous solution