GE17-5281-01
GSTrap™ High Performance
Cytiva 17-5281-01, pack of 5 × 1 mL
About This Item
Recommended Products
ligand
glutathione
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5281-01
parameter
42 psi (H2O at room temperature.)
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
3-12
working range
3-12
capacity
>7 mg/mL binding capacity(H2O at room temperature.)
Related Categories
General description
Application
GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.
Features and Benefits
- High resolution purification of GST-tagged Proteins.
- Mild elution conditions preserving Protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Storage and Stability
Analysis Note
Other Notes
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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