GE17-5130-01
GSTrap™ Fast Flow
Cytiva 17-5130-01, pack of 5 × 1 mL
Synonym(s):
GSTrap column
About This Item
ligand
glutathione and 10-carbon linker arm
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5130-01
parameter
flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
3-12
working range
3-12
capacity
>10 mg/mL binding capacity
General description
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
* See the section for licensing information in our terms and conditions.
Features and Benefits
- Fast and simple one-step purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast Flow provides good flow properties.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Protocols
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
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