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G2150

Sigma-Aldrich

L-Glutamine solution

200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

Synonym(s):

L-Glutamine for cell culture

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About This Item

CAS Number:
56-85-9
MDL number:
MFCD00008044
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24895088
NACRES:
NA.75

grade

Hybri-Max™

Quality Level

300

sterility

sterile-filtered

assay

≥99% (TLC)

form

solution

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

color

clear colorless

shipped in

dry ice

storage temp.

−20°C

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

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General description

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Application

The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.

Preparation Note

Prepared in cell culture grade water.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of human antibodies from transgenic mice.
C Geoffrey Davis et al.
Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
Abraham L Moure et al.
Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Glutamine and antioxidants in critically ill patients.
Daren Heyland et al.
The New England journal of medicine, 369(5), 484-485 (2013-08-02)
A U Lekmanov et al.
Anesteziologiia i reanimatologiia, (1)(1), 49-51 (2013-07-03)
Comparing analysis of randomized study was conducted. 40 Pediatric patients (2-15 years old) with heavy thermic burns and concomitant injuries were recruited into the study to assess the glutamine solution intravenous use efficiency. Parenteral feeding and feeding with glutamine solution
Ethan B Butler et al.
Cancer research, 73(9), 2709-2717 (2013-04-24)
Cancer cells are markedly different from normal cells with regards to how their metabolic pathways are used to fuel cellular growth and survival. Two basic metabolites that exemplify these differences through increased uptake and altered metabolic usage are glucose and
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Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

Chromatograms

application for HPLC

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