F3651

Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

≥90% (HPLC)

Synonym(s):
FITC, Fluorescein isothiocyanate
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
Beilstein/REAXYS Number:
1407295
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥90% (HPLC)

form

powder

application(s)

hematology: suitable
histology: suitable

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein 5(6)-isothiocyanate has been used:
  • for fluorescence labeling of bacteria
  • as a standard for calibration of fluorometer
  • for the labeling of exosomes
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Packaging

100 mg in glass bottle

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

hazcat

Resp. Sens. 1

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

Thymic exosomes promote the final maturation of thymocytes.
Lundberg V
Scientific Reports, 6, 36479-36479 (2016)
Handbook of Affinity Chromatography (2005)
Effects of supplemental 25-hydroxycholecalciferol on growth performance, small intestinal morphology, and immune response of broiler chickens.
Chou SH
Poultry Science, 88, 2333-2333 (2009)
Characterization of proteolytic enzyme activities in macroalgae.
Perez-Llorens JL
European Journal of Phycology, 38, 55-64 (2003)
Hermanson GT
Bioconjugate Techniques (2013)

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