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EPI001

Sigma-Aldrich

Histone Acetyltransferase (HAT) Activity Assay Kit

100 assays in 96 well plates

Enzyme Commission number:
NACRES:
NA.41

Quality Level

usage

100 assays in 96 well plates

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.

Features and Benefits

  • Sensitive and reliable assay
  • Utilizes colorimetric methods
  • Sample type: cell and tissue lysates
  • Species reactivity: mammalian
  • Suitable for individual tests or high throughput assays and kinetic studies
  • Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
  • Convenient 96-well microplate format
  • Continuously measures HAT activity; suitable for kinetic studies
  • No HDAC interference; crude nuclear extract can be used directly

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Certificate of Origin

Christian Koehler et al.
The Journal of biological chemistry, 289(13), 8989-8999 (2014-02-11)
The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex is a transcription coactivator that contains a histone H2B deubiquitination activity mediated by its Ubp8 subunit. Full enzymatic activity requires the formation of a quaternary complex, the deubiquitination module (DUBm) of SAGA, which is composed...
Jian Zhong et al.
Cancer research, 74(6), 1870-1880 (2014-02-01)
Overexpression of the histone acetyltransferase p300 is implicated in the proliferation and progression of prostate cancer, but evidence of a causal role is lacking. In this study, we provide genetic evidence that this generic transcriptional coactivator functions as a positive...
Atsuhito Nakao
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2153-2157 (2014-01-21)
IgE-mediated immediate-type allergic reactions in the skin show a diurnal rhythm, although the regulatory mechanisms are poorly understood. In mammals, the circadian rhythms are driven by the circadian clock system consisting of the light-entrained central clock located in the suprachiasmatic...
Shigeki Shimba
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2119-2123 (2014-01-21)
Several epidemiological studies have suggested that the perturbation of circadian rhythm has adverse metabolic consequences (e.g., dyslipidemia) in humans. At the molecular level, circadian rhythms are encoded by an autoregulatory loop composed of a set of transcription activators (BMAL1/CLOCK) that...
Koji Maemura
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2124-2129 (2014-01-21)
Cardiovascular function exhibits circadian oscillation. Furthermore, the onset time and severity of cardiovascular diseases exhibit diurnal variation. The internal circadian clock whose center exists in the suprachiasmatic nucleus regulates this circadian variation. Many studies have reported that night shift workers...

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