ECM002
Thrombospondin-1 human
recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
THBS, THBS1, TSP, TSP1, Thrombospondin-1
About This Item
Recommended Products
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
sterility
sterile
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
127.5 kDa (The protein migrates as a 140 kDa band on SDS-PAGE due to glycosylation.)
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg endotoxin, tested
solubility
water: soluble
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... THBS1(7057)
General description
Application
- To treat hepatocyte (IHH) cancer cells to study the role of aspartyl protease 1 (MfSAP1) in extracellular matrix degradation.
- In in vitro Extracellular Matrix (ECM) protein degradation assays.
- In rat C6 astroglioma cells, for coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression.
- In the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.1-25 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Biochem/physiol Actions
Features and Benefits
- Human-derived thrombospondin
- Produced through recombinant expression in HEK 293 cells
- Low endotoxin levels
Physical form
Analysis Note
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
Attachment Factors for 3-Dimensional Cell Culture
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
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