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D9944

Sigma-Aldrich

Monoclonal Anti-DYNLT1 antibody produced in mouse

~1.0 mg/mL, clone T1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CW-1, Anti-Dynein, light chain, Tctex-type 1, Anti-TCTEL1, Anti-Tctex-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~12.5 kDa

species reactivity

monkey, mouse, bovine, human, rat

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
western blot: 8-16 μg/mL using NRK total cell lysate

isotype

IgG1

UniProt accession no.

P63172

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DYNLT1(6993)
mouse ... Dynlt1(21648)
rat ... Dynlt1(83462)

Immunogen

recombinant dynein 1 light chain-myosin basic protein fusion protein.

Application

Monoclonal Anti-DYNLT1 antibody produced in mouse is suitable for immunocytochemistry and immunoblotting at a working concentration of 8-16μg/mL using NRK total cell lysate.

Biochem/physiol Actions

Cytoplasmic dynein motor complex is responsible for minus-end, microtubule-based motile processes. It is thus involved in a wide range of eukaryotic cellular processes including trafficking of membranal vesicles, viruses and other intracellular particles. DYNLT1 is a light chain subunit of the complex that is involved in cargo binding. It binds specifically to the cytoplasmic tail of rhodopsin and links rhodopsin-bearing vesicles to the dynein motor complex for transport. The cargo is released by the disassembly of dynein complex upon phosphorylation of DYNLT1 on Ser82 residue. This process is critical for the apical delivery of membrane cargoes. It also mediates neurite extension by modulating actin dynamics and also Rac1 activity. The abundance and expression pattern of DYNLT1 in neuronal tissues may also contribute to the invasion capabilities of neurotropic viruses like poliovirus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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N Hirokawa
Science (New York, N.Y.), 279(5350), 519-526 (1998-02-07)
Cells transport and sort proteins and lipids, after their synthesis, to various destinations at appropriate velocities in membranous organelles and protein complexes. Intracellular transport is thus fundamental to cellular morphogenesis and functioning. Microtubules serve as a rail on which motor
Steffen Mueller et al.
The Journal of biological chemistry, 277(10), 7897-7904 (2001-12-26)
The cellular receptor for poliovirus CD155 (or PVR) is the founding member of a new class of membrane-associated immunoglobulin-like proteins, which include the mouse tumor-associated antigen E4 (Tage4) and three proteins termed "nectins." Using a yeast two-hybrid screen we have
A W Tai et al.
The Journal of cell biology, 153(7), 1499-1509 (2001-06-27)
Despite the existence of multiple subunit isoforms for the microtubule motor cytoplasmic dynein, it has not yet been directly shown that dynein complexes with different compositions exhibit different properties. The 14-kD dynein light chain Tctex-1, but not its homologue RP3
Ting-Yu Yeh et al.
Traffic (Copenhagen, Denmark), 7(11), 1495-1502 (2006-09-08)
Post-Golgi to apical surface delivery in polarized epithelial cells requires the cytoplasmic dynein motor complex. However, the nature of dynein-cargo interactions and their underlying regulation are largely unknown. Previous studies have shown that the apical surface targeting of rhodopsin requires
Jacques Brocard et al.
The European journal of neuroscience, 46(11), 2754-2767 (2017-11-03)
MAP6 proteins were first described as microtubule-stabilizing agents, whose properties were thought to be essential for neuronal development and maintenance of complex neuronal networks. However, deletion of all MAP6 isoforms in MAP6 KO mice does not lead to dramatic morphological
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