Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture





cell culture | mammalian: suitable


glucose: high
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in


storage temp.


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Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".


Formulated to contain 13.4 grams of powder per liter of medium.


Supplement with 3.7 g/L sodium bicarbonate.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Ana M S Cardoso et al.
Human molecular genetics, 28(16), 2738-2751 (2019-05-16)
Glioblastoma (GB) is the most aggressive and common form of primary brain tumor, characterized by fast proliferation, high invasion, and resistance to current standard treatment. The average survival rate post-diagnosis is only of 14.6 months, despite the aggressive standard post-surgery treatment...
Cindy C Shu et al.
Arthritis & rheumatology (Hoboken, N.J.), 68(4), 868-879 (2015-12-05)
To investigate the role of the heparan sulfate (HS) proteoglycan perlecan (HSPG-2) in regulating fibroblast growth factor (FGF) activity, bone and joint growth, and the onset and progression of posttraumatic osteoarthritis (OA) in a mouse gene-knockout model. Maturational changes were...
Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.
Abulizi, et al.
Cell Metabolism, 32, 751-766 (2021)
Lingling Lu et al.
Acta histochemica, 121(6), 695-703 (2019-06-27)
Renal fibrosis is a common mechanism that leads to all kidney diseases and Epithelial-mesenchymal transition (EMT) is considered as one of the potential mechanisms of renal fibrosis. Inhibitor of growth 4 (ING4) was reported to involve in several diseases; especially...
Ayako Sasaki et al.
Human cell, 29(1), 37-45 (2015-11-11)
Of two stereoisomers of glucose, only D- and not L-glucose is abundantly found in nature, being utilized as an essential fuel by most organisms. The uptake of D-glucose into mammalian cells occurs through glucose transporters such as GLUTs, and this...
Cell culture protocol for passaging and splitting suspension cell lines
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Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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