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Key Documents

CL6B200

Sigma-Aldrich

Sepharose CL-6B Size Exclusion Resin

Cross-linked, 90-350 mesh

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.84

product name

Sepharose CL-6B, Cross-linked

Quality Level

form

suspension

bead diameter

40-165 μm

pore size

10,000-1,000,000 fractionation range (Dextrans)
10,000-4,000,000 fractionation range (Globular proteins)

storage temp.

2-8°C

General description

Sepharose CL is a cross-linked derivative of sepharose, prepared by reacting sepharose with 2,3-dibromopropanol under strongly alkaline conditions.

Application

Sepharose CL-6B is used in affinity chromatography, protein chromatography, gel filtration chromatography, separation media and resins. Sepharose CL-6B has been used to study in vitro inhibition of human melanoma cells and to gather data in other valuable anticancer studies.
SepharoseCL-6B has been used for the purification of glucoamylase enzyme and targeted ribonucleoprotein (RNP).

Legal Information

Sepharose is a trademark of Cytiva

Replaced by

Product No.
Description
Pricing

pictograms

Flame

signalword

Danger

hcodes

Hazard Classifications

Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3


Certificates of Analysis (COA)

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Hiroshi Tomoda et al.
Analytical biochemistry, 311(1), 50-56 (2002-11-21)
The galabiose structure Galalpha1-4Gal is rarely found in natural glycoproteins, but is abundantly present in pigeon egg white proteins as Galalpha(1-4)Galbeta(1-4)GlcNAc termini. Pigeon ovalbumin, ovomucoid, or the whole egg white were immobilized on periodate-oxidized Sepharose CL-6B gels by reductive amination.
Carla Cruz et al.
Journal of molecular recognition : JMR, 24(6), 975-980 (2011-11-01)
The binding between four matrices (beaded cellulose, cellulose acetate, cellulose triacetate and Sepharose CL-6B) and beaded cellulose derivatized with a thiacarbocyanine dye with 5'-mononucleotides is investigated by Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR) technique. This procedure intends to identify
Mrinal Kumar Das et al.
International journal of biological macromolecules, 49(5), 1096-1103 (2011-09-21)
Articulatin-D, a 66 kDa ribosome inactivating protein (RIP) comprised of 29 kDa A-chain linked to 35 kDa B-chain, is purified from leafless mistletoe (Viscum articulatum) parasitic on Dalbergia sp. from Western Ghats (India). N-terminal sequence and LC-MS/MS analyses of A-
Preparation and Mammalian Plasma Membranes, 7 (1979)
N Vinay Kumar et al.
Methods (San Diego, Calif.), 32(4), 389-397 (2004-03-09)
Intracellular signaling by protein kinases controls many aspects of cellular biochemistry and physiology. Determining the direct substrates of protein kinases is important in understanding how these signaling enzymes exert their effect on cellular functions. One of the recent developments in

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